DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression

Figure 3

Knockdown of DNM3OS decreased the apoptosis and senescence of spermatogonia induced by ADR treatment. GC-1 spg cells were transfected with si-DNM3OS or si-NC, followed by 24 hr of incubation with 0.5 μM ADR. (a) The levels of DNM3OS and miR-214-5p expression were determined by quantitative real-time PCR. (b) Cellular activity was determined by the CCK-8 assay. (c) EdU incorporation assays were performed to assess cell proliferation ability. The actual length of the scale in the images is 50 μm. (d) Apoptotic cells were identified by flow cytometry with Annexin V-PI double staining. (e) Senescence-associated β-galactosidase (SA-β-gal) staining was used to evaluate cell senescence. The actual length of the scale in the images is 50 μm. Data represent the mean value ± SD. ^### represents si-NC vs. si-NC + ADR; , represents si-NC + ADR vs. si-DNM3OS + ADR.

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