METTL16 Inhibits the Malignant Progression of Epithelial Ovarian Cancer through the lncRNA MALAT1/β-Catenin Axis

Figure 2

Effect of METTL16 on cell proliferation, migration, and invasion of OC cells. (a) Relative mRNA level of METTL16 in five OC cell lines (OVCAR3, SKOV3, HO8910, ES-2, and A2780), compared with human epithelial ovarian cell line (IOSE80). (b) Relative mRNA and protein expression levels of METTL16 72 hr after SKOV3 and OVCAR3 cells were transfected with sh-METTL16 (sh-METTL16-1 or sh-METTL16-2) or their control sh-RNA (sh-NC); relative mRNA and protein expression levels of METTL16 72 hr after A2780 cells were transfected with oe-METTL16 or oe-METTL16 control (oe-NC). (c, d) Detection of cell proliferation by EdU assay of SKOV3 and OVCAR3 cells after they were transfected with sh-METTL16 (sh-METTL16-1 or sh-METTL16-2) or their control sh-RNA (sh-NC) as well as A2780 cells after they were transfected with oe-METTL16 or oe-METTL16 control (oe-NC) (scale bar, 100 μm). (e, f) Detection of invasion (e) and migration (f) of SKOV3 and OVCAR3 cells that were transfected with sh-METTL16 (sh-METTL16-1) or their control sh-RNA (sh-NC). ns: , , , and .

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