Research Article

Mitochondria-Associated Gene SLC25A32 as a Novel Prognostic and Immunotherapy Biomarker: From Pan-Cancer Multiomics Analysis to Breast Cancer Validation

Figure 10

SLC25A32 promotes EMT in breast cancer. (a) Transwell assays were used to detect the invasion of the two groups of cells. (b) The migration ability of 231 cell lines was detected using scratch tests at 0, 24, and 48 hr, respectively. The migration rate at a certain time = (0 hr scratch area − scratch area at a certain time)/0 hr scratch area × 100%. (c) The migration ability of 549 cells was determined using the same method, and the bar graph illustrates the migration rate. (d, e) Western blot was used to detect the expression changes of EMT marker proteins in 231 and 549 cell lines after transient SLC25A32 knockdown. The blots were cropped from the full-length original blot to improve the clarity and conciseness of the presentation. ; ; .
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