Astragaloside IV Protects against Shear Stress-Induced Glycocalyx Damage and Alleviates Abdominal Aortic Aneurysm by Regulating miR-17-3p/Syndecan-1

Figure 6

AS-IV mitigates shear stress-induced damage in HAECs via miR-17-3p inhibition. HAECs were divided into four groups: control, SS (5 dyn/cm² shear stress), SS + AS-IV (100 μmol/L), and SS + AS-IV + miR-17-3p. (a) Comparison of miR-17-3p expressions in each group. (b–d) Comparison of SDC1 mRNA and protein expressions in each group. (e,f) Comparison of cell apoptosis rates, including early and late apoptosis, in each group. Early apoptosis was observed in the B4 quadrant, and late apoptosis was observed in the B2 quadrant. (g) Immunofluorescence staining revealed the CS expression level to assess the glycocalyx damage. , vs. Control group; ^#, ^## vs. SS group; ^{^^}, ^{^^^} vs. SS + AS-IV group. AS-IV, astragaloside IV; HAECs, human aortic endothelial cells; SS, shear stress; and CS, chondroitin sulfate.

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