miR-224-5p Attenuates Allergic Responses in Mice with Allergic Rhinitis by Modulating the Th1/Th2 Response

Figure 2

Overexpression of miR-224-5p attenuates the AR process: (a) RT-qPCR for measuring miR-224-5p transfection efficiency, (b) ELISA for measuring the levels of IFN-γ, IL-4, IL-5, and IL-13, (c) flow cytometry for detecting Th1 differentiation, (d) flow cytometry for detecting Th2 differentiation, (e) RT-qPCR for testing the miR-224-5p level, (f) observation of the frequency of sneezing, (g) observation of the frequency of nasal friction, (h) ELISA for assessing the levels of OVA-specific IgE, IFN-γ, IL-4, IL-5, and IL-13, (i) flow cytometry for detecting Th2 differentiation in mouse spleen, (j) HE staining for observing histopathological alterations of mouse nasal mucosa (scale bar = 100 µm), and (k) PAS staining for observing the histopathological alterations of nasal mucosa (scale bar = 100 µm). , vs. the NC group or normal group; ^$$$ vs. the AR group.

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