miR-224-5p Attenuates Allergic Responses in Mice with Allergic Rhinitis by Modulating the Th1/Th2 Response

Figure 5

miR-224-5p moderates AR symptoms through GATA3 regulation of Th1/Th2 responses: (a) western blot or testing the GATA3n level in mouse nasal mucosa, (b) RT-qPCR for testing the miR-224-5p level in mouse nasal mucosa, (c) RT-qPCR for testing the miR-224-5p level in mouse spleen, (d, e) observation of the frequency of sneezing and nasal friction, (f) flow cytometry for testing the Th2 differentiation in mouse spleen, (g) ELISA for testing the levels of OVA-specific IgE, IFN-γ, IL-4, IL-5, and IL-13 in mouse serum; (h, i) HE and PAS staining for observing histopathological alterations of mouse nasal mucosa (scale bar = 100 µm), and (j) HE staining for observing the histopathological alterations of spleen (scale bar = 100 µm). vs. the normal group; ^$$, ^$$$ vs. the AR group; ^### vs. the AR + miR-224-5p mimic group.

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