Hsa_circ_0000190 Promotes NSCLC Cell Resistance to Cisplatin via the Modulation of the miR-1253/IL-6 Axis

Figure 3

Hsa_circ_0000190 functions as a molecular sponge capable of sequestering miR-1253. (a) qPCR was used to assess the levels of nine miRNA candidates in NSCLC patient tumors and paracancerous tissues. (b) qPCR was used to assess miR-1253 levels in A549/DDP and H460/DDP cells following si-NC or si-hsa_circ_0000190 transfection. (c) The identified sequence overlap between hsa_circ_0000190 and miR-1253. (d, e) The ability of hsa_circ_0000190 and miR-1253 to interact was assessed through dual-luciferase reporter assays (d) and RIP assays (e). (f) miR-1253 levels were assessed via qPCR in NSCLC tumor tissues harvested from DDP-resistant (n = 73) and DDP-sensitive (n = 63) patients. (g) Spearman’s correlations were used to assess linear relationships between hsa_circ_0000190 and miR-1253 levels in DDP-resistant NSCLC tissues. (h) qPCR was used to detect miR-1253 in the indicated NSCLC and control cell lines. (i) qPCR was used to quantify hsa_circ_0000190 transfection efficiency. (j) miR-1253 expression in A549/DDP and H460/DDP was quantified via qPCR following vector, hsa_circ_0000190, si-NC, or si-hsa_circ_0000190 transfection. and .

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