Research Article
Downregulated PDIA3P1 lncRNA Impairs Trophoblast Phenotype by Regulating Snail and SFRP1 in PE
Figure 4
SFRP1 as a downstream target of PDIA3P1. (a, b) Identification of differentially expressed genes in siPDIA3P1-transfected HTR-8/SVneo cells through RNA-sequencing and gene expression profiling. (c) Gene ontology analysis revealed significant effects of PDIA3P1 depletion on cell growth and adhesion processes in trophoblasts. (d, e) RT-PCR confirmation of altered mRNA gene levels in PDIA3P1-deficient HTR-8/SVneo and JAR cells. (f) Western blot analysis showing changes in SFRP1 and β-catenin expression after siNC or si-PDIA3P1 transfection in HTR-8/SVneo and JAR cells, with β-actin as the internal control. (g) RT-qPCR analysis of SFRP1 mRNA expression in PE and normal placental tissues. (h) Western blot analysis of SFRP1 protein levels in PE and normal placental tissues, using GAPDH as a reference gene. (i) IHC demonstrates higher SFRP1 expression in PE placental tissues compared to normal tissues. Data are presented as mean ± SD. ; .
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |
(i) |