Development of the RP-HPLC Method for Simultaneous Determination and Quantification of Artemether and Lumefantrine in Fixed-Dose Combination Pharmaceutical Dosage Forms

Table 4

Precision study data for artemether and lumefantrine: repeatability and intermediate precision.

Concentration (μg/mL) at 100%	Repeatability	Intermediate precision
	Peak area (mAU.s)	Peak area (mAU.s)
Lumefantrine
	9465.165	9474.324
1100	9476.799	9501.247
1100	9422.657	9482.271
1100	9450.740	9356.301
1100	9456.740	9468.233
1100	9439.310	9430.342
Mean	9451.9018	9452.1197
SD	19.153865	52.403728
% RSD	0.20	0.55
Artemether
600	252.641	253.100
600	252.541	252.341
600	252.642	252.603
600	252.583	251.712
600	251.524	250.789
600	252.166	251.342
Mean	252.3495	251.981
SD	0.44200577	0.8568
% RSD	0.18	0.34

Acceptance criteria: RSD ≤ 2%.