Development of the RP-HPLC Method for Simultaneous Determination and Quantification of Artemether and Lumefantrine in Fixed-Dose Combination Pharmaceutical Dosage Forms

Table 5

Accuracy study.

API	Accuracy level (%)	Amount added (μg/mL)	Amount recovered (μg/mL)	% recovery	Mean % recovery	% RSD
Artemether	80	7945	7955	100.13	99.79	0.01
		8123	8098	99.69
		8057	8022	99.57
	100	10039	10032	99.93	99.93	0.00
		10041	10039	99.98
		10043	10032	99.89
	120	11950	12054	100.87	100.16	0.01
		12073	12045	99.77
		12004	11985	99.84
Lumefantrine	80	9709	9695	99.86	99.50	0.51
		9692	9587	98.92
		9714	9688	99.73
	100	12143	12062	99.33	99.33	0.22
		12140	12098	99.65
		12133	12103	99.75
	120	14556	14401	98.94	99.04	0.09
		14553	14423	99.11
		14556	14421	99.07

Acceptance criteria: RSD ≤ 2%.