Development of the RP-HPLC Method for Simultaneous Determination and Quantification of Artemether and Lumefantrine in Fixed-Dose Combination Pharmaceutical Dosage Forms

Table 8

Results of the greenness study.

Greenness study components	Factors considered	Strategies employed	Measure of success for greenness study on a scale of 0–10
Solvent selection	Lower toxicity renewable resources	Low-toxic solvents were used (acetonitrile and orthophosphoric acid)	8
Less solvent consumption	Utilize small quantities of solvents	A small volume (0.5 mL) of orthophosphoric acid was dissolved in 1000 mL of deionized water	9
Waste generation	Minimize waste effective use of resources	Required quantities were calculated before measuring to avoid waste	9
Energy consumption	Use of energy-efficient instrument	Low energy consumption (efficient) instruments were used in the study	8
Simple size and resource efficiency	Minimize sample size	A small sample size was utilized	9
Life cycle assessment	Assess the entire life cycle impact	Ensured a comprehensive understanding of the environmental impact	9
Biodegradability and environmental friendliness	Selection of biodegradable and environmentally friendly chemicals	All chemicals used were environmentally friendly and fast biodegradation	9
Compliance with green chemistry	Tests should comply with green chemistry principles	Green chemistry principles ensured	9
Reduction in analysis time	Short experimental/reaction time	Short run time in HPLC analyses. Use of a sonicator to speed up dissolution	9

(0): no success, (1–3): low success, (4–6): moderate success, (7-8): high success, and (9-10): very high success.