Ethanol Extract of Ampelopsis brevipedunculata Rhizomes Suppresses IgE-Mediated Mast Cell Activation and Anaphylaxis

Figure 3

Effects of ABE on degranulation and intracellular calcium influx in mast cells. (a) RBL-2H3 were incubated with ABE for 12 h. Cell viability was determined using MTT assay and revealed as the relative absorbance of control cells and ABE treated cells. (b–d, f–h) Anti-DNP IgE-sensitized mast cells were pretreated with/without drugs (ABE or Dex) for 1 h. The cells were stimulated with DNP-HSA for 4 h (RBL-2H3) or 30 min (BMMCs and RPMCs). The released histamine was purified and detected using a fluorescent plate reader. The percentage of β-hexosaminidase release was calculated. (e) Anti-DNP IgE-sensitized RBL-2H3 were incubated with Fluo-3/AM for 1 h, treated with/without ABE or BAPTA-AM for 1 h, and challenged with DNP-HSA. Graph data represent the relative intracellular calcium levels compared with those of control cells. The graph data represent the mean ± SEM. compared with the DNP-HSA-stimulated group. Dex: dexamethasone.

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