Truncated MTA-1: A Pitfall in ELISA-Based Immunoassay of HTLV-1 Infection
Figure 1
Alw 441 restriction analysis
of plasmids, extracted from transformed clones. Plasmids of transformed clones are visualized on ethidium
bromide-stained 1% agarose gel after purification and digestion with Alw 441. Lane kb DNA Ladder; Lane 2, 4: digested plasmid from transformed clones with
no insert using Alw 441 restriction enzyme; Lane 3, a transformed clone containing
pET-22b(+) with a 455 bp MTA-1 insert; Lane 5 indicates Alw 441 restriction
map of nonrecombinant pET-22b(+) (negative control).
