Dihydroartemisinin Sensitizes Mutant p53 (R248Q)-Expressing Hepatocellular Carcinoma Cells to Doxorubicin by Inhibiting P-gp Expression

Figure 1

p53 (R248Q) induced P-gp expression and ADM resistance in Hep3B cells. (a) Western blot analysis for the expression of p53 and P-gp in Hep3B-derived clones. Null: Hep3B, Vector: empty vector lenti-virus-infected Hep3B cells, WT : Hep3B cells expressing WT p53, and R248Q : Hep3B cells expressing p53 (R248Q). (b) Quantitative analysis of P-gp in Hep3B-derived clones. (c) Detection of cell viability upon ADM treatment by MTT assays. (d) Representative image of colony formation. (e) Quantitative graph of colony numbers. (f) Detection of cell apoptosis by flow cytometry. (g) Quantitative graph of apoptotic rates. (h) Morphological changes of cells (magnification ×400). The red arrowhead indicated membrane invagination, nuclear condensation, and vacolation in apoptotic cells. The data represent the mean ± SD of 3 independent experiments. For (c), and compared with the WT p53 group. , , and compared with the control group. For (b), (e), and (g), and .

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