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Biochemistry Research International
/
2011
/
Article
/
Tab 3
/
Review Article
Bioseparation of Four Proteins from
Euphorbia characias
Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase
Table 3
Purification of
Euphorbia
latex purple acid phosphatase.
Step
Total protein (mg)
Total activity (nkat)
Specific activity (nkat/mg)
Yield (%)
Purification (fold)
Acetone powder crude extract
3500
418567
119.6
100
1
Ammonium sulfate fractionation
534
165133
309
39.4
2.6
DEAE–cellulose chromatography
101
92700
917.8
22
7.6
Gel filtration chromatography
5.9
58567
9927
14
83