Research Article
The Binding Affinity of Small Molecules with Yam Tyrosinase (Catechol Oxidase): A Biophysical Study
Table 1
Interaction kinetics of yam tyrosinase with different inhibitors fit to a two-state model using Biacore evaluation software.
| | Compounds | ka1 (1/ms) | kd1 (1/s) | ka2 (1/s) | kd2 (1/s) | KD (M) |
| | Kojic acid | 334.3 ± 16 | 0.03182 ± 0.0013 | 5.39 × 10−3 ± 7.8 × 10−5 | 0.03877 × 10−3 ± 6.5 × 10−4 | 6.39 × 10−6 | | Hydroquinone | 2.893 ± 0.13 | 0.9166 ± 0.02 | 1.569 × 10−3 ± 3.5 × 10−5 | 5.185 × 10−3 ± 1.3 × 10−4 | 0.2432 | | Crocin | 224.3 ± 11 | 0.5772 ± 0.025 | 3.525 × 10−3 ± 9.7 × 10−5 | 6.429 × 10−3 ± 1.4 × 10−4 | 0.001662 | | L-DOPA | 9.54 × 104 | 0.5089 ± 0.085 | 0.001544 ± 1.50 × 104 | 5.23 × 10−4 ± 8.40 × 10−5 | 1.35 × 10−6 |
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