Anlotinib Inhibits Cisplatin Resistance in Non-Small-Cell Lung Cancer Cells by Inhibiting MCL-1 Expression via MET/STAT3/Akt Pathway

<div>Effects of oe-MET and shMCL-1 on anlotinib-mediated DDP resistance in NSCLC cells. (a, b) A549/DDP and H1299/DDP cells were treated with 1 <i>μ</i>M anlotinib, 2 <i>μ</i>M DDP, or 1 <i>μ</i>M anlotinib+2 <i>μ</i>M DDP. (a) MET and MCL-1 mRNA expression was determined by qRT-PCR. (b) The p-MET/MET and MCL-1 protein expression was examined by western blot analysis. (c–g) A549/DDP and H1299/DDP cells were transfected with oe-MET-1 and sh-MCL-1, followed by treatment with 1 <i>μ</i>M anlotinib and 2 <i>μ</i>M DDP. (c) qRT-PCR was performed to examine MET and MCL-1 mRNA expression. (d) Western blot analysis was carried out to detect p-MET/MET and MCL-1 protein expression. 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Canadian Respiratory Journal

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Figure 4

Figure 4: Anlotinib Inhibits Cisplatin Resistance in Non-Small-Cell Lung Cancer Cells by Inhibiting MCL-1 Expression via MET/STAT3/Akt Pathway 