Anlotinib Inhibits Cisplatin Resistance in Non-Small-Cell Lung Cancer Cells by Inhibiting MCL-1 Expression via MET/STAT3/Akt Pathway

<div>Effects of oe-MET and ACT001 on anlotinib-mediated DDP resistance in NSCLC cells. A549/DDP and H1299/DDP cells were transfected with oe-MET-1 and then treated with anlotinib, DDP, and ACT001. (a) The mRNA expression levels of MET and MCL-1 were assessed by qRT-PCR. (b) The protein levels of p-MET/MET, p-STAT3/STAT3, p-Akt/Akt, and MCL-1 were measured by western blot analysis. CCK8 assay (c), colony formation assay (d), and transwell assay (e) were performed to examine cell viability, proliferation, migration, and invasion. <svg height="10.7395pt" id="M12" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 56.4993 10.7395" width="56.4993pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 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Canadian Respiratory Journal

fig5

Figure 5

Figure 5: Anlotinib Inhibits Cisplatin Resistance in Non-Small-Cell Lung Cancer Cells by Inhibiting MCL-1 Expression via MET/STAT3/Akt Pathway 