Ferroptosis Mediates Pulmonary Fibrosis: Implications for the Effect of Astragalus and Panax notoginseng Decoction

Figure 4

APD reduces collagen deposition and fibrosis markers in pulmonary fibrosis: histological and molecular analyses. (a) Masson staining to compare the collagen deposition in lung tissues of each group. (b) Immunofluorescence to observe COL-I and the expression level of COL-III. (c) Positive area ratio for COL-I (green) and COLI-III (red) using the film of immunofluorescence. (d) RT-qPCR for relative expression of COL-I and COL-III in the lungs (n = 3). (e) Determination of the relative content of HYP in lung tissue using an alkaline water hydroxyproline kit (n = 5). (f) Lung tissue protein to detect the relative expression of α-SMA (n = 3). (g) RT-qPCR for relative expression of α-SMA in the lungs (n = 3).The data were obtained through three independent experiments. , vs. the control group; ^#, ^## vs. the model group.

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