Hypoxia-Inducible Factor-1α Regulates High Phosphate-Induced Vascular Calcification via Type III Sodium-Dependent Phosphate Cotransporter 1

Figure 2

Knockdown efficacy and cell growth curve. The expression levels of HIF-1α protein (a) and mRNA (b) were examined by western blot analysis and RT-qPCR on day 7 following HIF-1α siRNA transfection. An MTT assay was used to assess the cell growth curve under different conditions (c). The data shown are indicative of mean ± SD. ^# vs. CNT; vs. HPSI; vs. control. CNT, HASMCs cultured under normal conditions with 0.9 mM Pi; HP, HASMCs cultured with 2.5 mM Pi; HPSI, HIF-1α siRNA-transfected HASMCs cultured with 2.5 mM Pi; HPOE, HIF-1α overexpression plasmid-transfected HASMCs cultured with 2.5 mM Pi. HIF-1α, hypoxia-inducible factor 1 alpha; siRNA, small interfering RNA; RT-qPCR, reverse transcription-quantitative PCR; SD, standard deviation; CNT, control; HP, high Pi; HASMCs, human aortic smooth muscle cells; Pi, inorganic phosphorous; HPSI, siRNA and high Pi; HPOE, overexpression and high Pi; MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; OD, optical density.