Hypoxia-Inducible Factor-1α Regulates High Phosphate-Induced Vascular Calcification via Type III Sodium-Dependent Phosphate Cotransporter 1

Figure 3

The changes in the gene expression levels of HIF-1α and Pit-1. RT-qPCR analysis was employed to examine the relative mRNA expression levels of HIF-1α and Pit-1 on day 7. The data shown are indicative of mean ± SD. vs. CNT; ^# vs. HP. CNT, HASMCs cultured under normal conditions with 0.9 mM Pi; HP, HASMCs cultured with 2.5 mM Pi; HPSI, HIF-1α siRNA-transfected HASMCs cultured with 2.5 mM Pi; HPOE, HIF-1α overexpression plasmid-transfected HASMCs cultured with 2.5 mM Pi. HIF-1α, hypoxia-inducible factor 1 alpha; pit-1, type III sodium-dependent phosphate cotransporter 1; RT-qPCR, reverse transcription-quantitative PCR; SD, standard deviation; CNT, control; HP, high Pi; HASMCs, human aortic smooth muscle cells; Pi, inorganic phosphorous; HPSI, siRNA and high Pi; HPOE, overexpression and high Pi.