Hypoxia-Inducible Factor-1α Regulates High Phosphate-Induced Vascular Calcification via Type III Sodium-Dependent Phosphate Cotransporter 1

Figure 5

Changes in cell phenotype. The relative mRNA expression levels of Runx2 and SM22α on day 7 were detected to trace the phenotypic transition of cultured HASMCs by RT-qPCR analysis. The data shown are indicative of mean ± SD. vs. CNT; vs. CNT; ^# vs. HP; ^## vs. HP. CNT, HASMCs cultured under normal conditions with 0.9 mM Pi; HP, HASMCs cultured with 2.5 mM Pi; HPSI, HIF-1α siRNA-transfected HASMCs cultured with 2.5 mM Pi; HPOE, HIF-1α overexpression plasmid-transfected HASMCs cultured with 2.5 mM Pi. Runx2, runt-related transcription factor 2; SM22α, smooth muscle 22 alpha; HASMCs, human aortic smooth muscle cells; RT-qPCR, reverse transcription-quantitative PCR; SD, standard deviation; CNT, control; HP, high Pi; Pi, inorganic phosphorous; HPSI, siRNA and high Pi; HIF-1α, hypoxia-inducible factor 1 alpha; HPOE, overexpression and high Pi.