Hangeshashinto Inhibits Porphyromonas gingivalis Pathogen-Associated Molecular Patterns-Mediated IL-6 and IL-8 Production through Toll-Like Receptors in CAL27 Cells

Figure 2

(a) Schematic representation of a reporter plasmid phIL-6pro-Luc containing the IL-6 promoter linked to the luciferase gene (luc). Putative binding sites of transcriptional factors are indicated. Plasmids of phIL-6pro-Luc and pRSV-β-gal (standard) were cotransfected into CAL27 cells. Twenty-four hours after transfection, the cells were cultured with hangeshashinto (b), resatorvid and C29 (c), and dexamethasone (d) for 24 hours. Then, the cells were stimulated with PAMP for 6 hours. The cells were lysed, and luciferase and β-galactosidase assays were performed. The transcriptional activities indicated fold values of those with no stimulators. Bars represent the means and range of triplicate samples. , versus stimulation with PAMP alone (one-way ANOVA with Tukey’s test).

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