Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus

Table 7

Positive detection rate for viral culture tests of 200 copies/mL for SARS-CoV-2, FluA, and FluB.

Virus	Subtype	Sample	Target	Batch 1 (%)	Batch 2 (%)	Batch 3 (%)
SARS-CoV-2	—	Sample 1	ORF	100	95	100
		N	100	100	100
		Sample 2	ORF	100	100	95
		N	100	95	100
		Sample 3	ORF	100	100	100
		N	95	100	100
FluA	H1N1	Sample 1	M	100	100	95
		Sample 2	M	95	100	100
		Sample 3	M	100	95	100
	H3N2	Sample 1	M	95	100	100
		Sample 2	M	100	100	100
		Sample 3	M	100	95	100
	H7N9	Sample 1	M	100	100	100
		Sample 2	M	100	95	100
		Sample 3	M	95	100	100
	H1N1 (2009)	Sample 1	M	100	100	100
		Sample 2	M	100	100	95
		Sample 3	M	100	100	100
	H5N1	Sample 1	M	100	95	100
		Sample 2	M	100	100	100
		Sample 3	M	100	100	100
FluB	Victoria	Sample 1	NS	100	100	95
		Sample 2	NS	100	100	100
		Sample 3	NS	95	100	100
	Yamagata	Sample 1	NS	100	95	100
		Sample 2	NS	100	100	100
		Sample 3	NS	95	100	100