Isolation of Female Germline Stem Cells from Mouse and Human Ovaries by Differential Adhesion

Figure 1

Isolation and culture of mouse female germline stem cells. (a) Schematic illustration of the culture system used for fGSC expansion. (b) Phase contrast images of mouse ovarian cell suspension after dissociation (A) and after 5 days of culture (B); spherical or grape-like clusters on day 30 in culture on MEF-coated plates (C, D; D at high magnification). (c) Viability percentage of mouse ovarian cells after ovarian tissue isolation by using PI staining analyzed by a BD FACS Aria II. (d) Characterization of established fGSCs. Expression of germ cell-specific markers in fGSCs detected by immunostaining analysis. The feeder cells were negative for these markers.

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