Mitotic Kinases Aurora-A, Plk1, and Cdk1 Interact with Elk-1 Transcription Factor through the N-Terminal Domain

Figure 3

In vitro kinase assay for Elk-1 phosphorylation by (a) Aurora-A (AurA), (b) Aurora-B (AurB), (c) Cdk1, (d) Plk1, and (e) Hexokinase 2 kinases. Recombinant Elk-1 protein was incubated with indicated kinases in the presence or absence of ATP, and phosphorylation was monitored by accumulation of ADP at 15, 30, 45, and 60 min time points as determined by increase in fluorescence (i.e., kinase activity was reported as relative fluorescence units). Reaction tube containing only reaction buffer was used as mock.

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