The Effect of Differentiation of Human Dental Pulp Stem Cells to Glial Cells on the Sensory Nerves of the Dental Pulp

Figure 5

Trigeminal neuron growth after adding conditioned medium: (a) trigeminal axon growth (TUBB3, red color; NF200, green color; DAPI, blue color) is shown by immunofluorescent staining in the presence of cell supernatant of SC-DPSCs; (b) CCK-8 was used to analyze the trigeminal neuron growth on different days after adding a conditioned medium of 6 days; (c) CCK-8 was used to analyze the trigeminal neuron growth on different days after adding a conditioned medium of 8 days; (d) comparison of trigeminal neuron growth after addition of 6 days induction medium and 8 days induction medium. Scale bar: 20 μm; SC-DPSCs, Schwann-like cells derived from dental pulp stem cells; NF-200, neurofilament 200; TUBB3, β-III tubulin; MAP2, microtubule-associated protein 2. .

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