Involvement of the Bacillus SecYEG Pathway in Biosurfactant Production and Biofilm Formation

Figure 4

The effect of Rose Bengal on SecYEG-mediated secretion of biosurfactant and biofilm formation. (a) Determination of the concentration of Rose Bengal in the growth and morphology of isolates. NM2: Bacillus isolate; RB: Rose Bengal. (b) PA_RBabs: Proteolytic activity in the absence of Rose Bengal and PA_RB: proteolytic activity in the presence of Rose Bengal. (c) CA_RBabs: Cellulolytic activity in the absence of Rose Bengal and CA_RB: cellulolytic activity in the presence of Rose Bengal. (d) AA_RBabs: Amylolytic activity in the absence of Rose Bengal and AA_RB: amylolytic activity in the presence of Rose Bengal. (e) BP_RBabs: Biosurfactant production in the absence of Rose Bengal and BP_RB: biosurfactant production in the presence of Rose Bengal. (f) BP_RBabs: LB biofilm production in the absence of Rose Bengal in LB and BP_RB: LB biofilm production in the presence of rose Bengal in LB. (g) CV_RBabs: Crystal violet assay of biofilm formation in the absence of Rose Bengal and CV_RB: crystal violet assay of biofilm formation in the presence of Rose Bengal.

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