Full-Length Genome Sequencing and Analysis of Hepatitis B Viruses Isolated from Iraqi Patients

Table 1

Primers used for amplification of the whole HBV genome.

Primer	Sequence (5′–3′)	Genome Location	Region	Ta (°C)	Amplicon size (bp)	Reference
F1	CAT​ACT​GCG​GAA​CTC​CTA​GC	1267–1286	X	61	681	Current study
R1	AGT​AAC​TCC​ACA​GTA​GCT​CC	1947–1928
F2	GAG​GCA​TAC​TTC​AAA​GAC​TG	1698–1717	PreC-C	59	826
R2	GAG​GGT​TAA​AGA​CAG​GTA​CAG	2523–2503
F3	GGA​AGA​GAA​ACG​GTC​ATA​GAG	2231–2251	P	60	941
R3	GGC​CTG​AGG​ATG​AGT​GTT​TC	3171–3152
F4	ATGGGGCAGAATCTTTCC	2848–2865	PreS1-S	59	1265	[11]
R4	CTG​TAT​GAT​GTG​ATC​TTG​TGG​C	930–909				Current study
F5	ATG​GAG​AAC​ATC​ACA​TCA​GG	155–174	P1	60	1540	[12]
R5	GTC​GGT​CGT​TGA​CAT​TAC​AG	1694–1675				Current study

Consensus DNA sequence created from aligning of 1094 genome sequences of HBV genotype D isolates found in the HBVdb, Ta: annealing temperature.