CD4⁺T and CD8⁺T Cells in Uterus Exhibit Both Selective Dysfunction and Residency Signatures

Figure 2

Expression of memory and residence markers on CD4⁺T cells and CD8⁺T cells from uterus and blood. Cells from uterus and blood were isolated and stained with anti-CD44, CD69, CD103, CD25, and CD27 mAbs. Gated on CD3⁺CD4⁺T (a, b) and CD3⁺CD8⁺T cells (c, d), and then compare the expression of CD44, CD69, CD103, CD25, and CD27 in uterus and blood. Representative dot plots and pie chart (n = 6) showed that gated on uterine CD3⁺CD4⁺CD44⁺ memory T cells (e) and CD3⁺CD8⁺T CD44⁺ memory T cells (f), and then detected the proportion of non-T_RM (CD69⁻CD103⁻) and T_RM (CD69⁺CD103⁺, CD69⁺CD103⁻, and CD69⁻CD103⁺) cells. Data were shown as mean ± SEM and compared with the Student’s t-test. NS, no significance; ; ; ; .

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