CD4⁺T and CD8⁺T Cells in Uterus Exhibit Both Selective Dysfunction and Residency Signatures

Figure 5

Uterine CD4⁺T and CD8⁺T cells highly expressed cytotoxic molecules and IFN-γ. Isolated cells from uterus were stained with phenotype markers, anti-GranzymeB, anti-CD107a, and Anti-IFN-γ mAbs. Representative and statistical charts showed that gated on CD3⁺CD4⁺T (a) and CD3⁺CD8⁺T cells (b), and then compared to the expression of cytotoxic molecules including GranzymeB and CD107a in uterus and blood. Isolated uterine cells were stimulated with or without PMA and Ionomycin in the existence of BFA for 6 hr to detect the expression of IFN-γ. The expression of IFN-γ on CD3⁺CD4⁺T (c) and CD3⁺CD8⁺T cells (d) were shown. Data were presented as mean ± SEM and compared with the Student’s t-test. NS, no significance; ; ; .

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