Bisphenol-A Abrogates Proliferation and Differentiation of C2C12 Mouse Myoblasts via Downregulation of Phospho-P65 NF-κB Signaling Pathway

Figure 5

Molecular mechanism of BPA in C2C12 myoblast proliferation and differentiation. C2C12 myoblasts were cultured in either growth medium (GM) or differentiation medium (DM) in the absence or presence of BPA at the indicated concentration for 72 h. Total proteins were extracted and subjected to Western blot analysis with anti-phospho-JNK, anti-phospho-p53, and anti-phospho-P65 NF-κB antibodies. The activation of each phosphorylated protein was expressed as fold change compared to the control group. ; ; compared to the control group. (a and c) Proliferation and (b and d) differentiation conditions.

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