NETosis Secondary to the Use of Levamisole-Adulterated Cocaine: A Likely Underlying Mechanism of Vasculopathy

Figure 1

Purity and viability of neutrophil-enriched suspensions. Circulating neutrophils from healthy controls were isolated by Ficoll-Hystopaque density gradient centrifugation followed by dextran sedimentation. Cell purity and viability were evaluated by labeling with anti-CD45-PE/Cy7 and anti-CD33-PE mAbs and PI/DiOC₆ staining, respectively. (a) Representative dot plots showing the strategy for gating CD44⁺CD33⁺ neutrophils and evaluating cell purity. (b) Representative dot plot illustrating the distinction of live cells (DiOC₆^bright IP^neg), cells with mitochondrial injury (DIOC₆^dimIP^neg), and cells with compromised plasma membrane (DIOC₆^negIP⁺⁺⁺). n = 4 independent experiments.

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