Detection of Intranasally Delivered Bone Marrow-Derived Mesenchymal Stromal Cells in the Lesioned Mouse Brain: A Cautionary Report
Figure 5
Ultrastructural analysis of striatal tissue from mice intranasally administered MSC-EGFPs. (a) Representative example of light microscopy image of DAB-stained tissue in striatum. (b) Characteristic structure observed in striatum of mice at 7 days after MSC-EGFP treatment. Darkened cell-like structures have few if any discernable organelles but are full of inclusion bodies, lipofuscin, and other degenerated products. A healthy nucleus and cell can be seen in the lower right portion of the panel (B′). 2 months after MSC-EGFP treatment, similar darkened structures were detected (c) as well as darkened cells with pyknotic nuclei, shrunken cytoplasm, and vacuoles (c) and (d). Scale bar is 2 μm.
