Nonviral Gene Delivery of Growth and Differentiation Factor 5 to Human Mesenchymal Stem Cells Injected into a 3D Bovine Intervertebral Disc Organ Culture System

Table 2

Primer list used for RT-PCR.

Abbreviation	Gene name	Forward	Reverse
Hs_18S	Reference gene	CGA TGC GGC GGC GTT ATT C	TCT GTC AAT CCT GTC CGT GTC C
Hs_ACAN	Aggrecan	CAT CAC TGC AGC TGT CAC	AGC AGC ACT ACC TCC TTC
Hs_COL1	Collagen1 A2	GTG GCA GTG ATG GAA GTG	CAC CAG TAA GGC CGT TTG
Hs_COL2A1	Collagen 2 A1	AGCAAGAGCAAGGAGAAG	GGGAGCCAGATTGTCATC
Hs_SOX9	SRY hox gene 9	GAG ACT TCT GAA CGA GAG	GGC TGG TAC TTG TAA TCC
Hs_KRT19	Keratin 19	TGT GTC CTC GTC CTC CTC	GCG GAT CTT CAC CTC TAG C
Hs_GDF5	Growth and differentiation factor 5 (GDF5)	ATCAGCATCCTCTTCATTGACTCT	ACACGACTCCACGACCAT
Hs_GFP	Green fluorescent protein	ATGACCAACAAGATGAAGAG	AAGTGGTAGAAGCCGTAG