Research Article

Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells

Table 1

Ingredients of the five passaging media conditions presented in this study.

Media nameBRL-C. pluscESC2i+cESC 2i+2i+ mod.

KO DMEM82%87%82%87%82%
FBS15%10%15%10%15%
Na Pyruvate (100 mM)1 mM1 mM1 mM1 mM1 mM
Glutamax (100x)2 mM1 mM1 mM1 mM1 mM
Nonessential amino acids (100x)1 mM1 mM1 mM1 mM1 mM
Beta-mercaptoethanol0.005 mM0.005 mM0.005 mM0.005 mM0.005 mM
Penicillin/Streptomycin (100x)1 mM1 mM1 mM1 mM1 mM
Nucleoside (100x)1 mM
LIF1000 U/mL1000 U/mL1000 U/mL50 U/mL
PD03259010.5 μM0.5 μM1 μM
CHIR990213 μM3 μM3 μM
A83-010.5 μM0.5 μM1 μM
rhIL61 ng/mL1 ng/mL
rhsILR6a1 ng/mL1 ng/mL
rmSCF1 ng/mL1 ng/mL
rhIGF11 ng/mL1 ng/mL
rhFGF21 ng/mL1 ng/mL

The media are all similar in a set of standard cell culture media ingredients (from KO DMEM to Penicillin/Streptomycin), but differ in the presence and concentrations of some of those ingredients, as well as LIF, 2i+ inhibitors (PDO325901, CHIR99021, and A83-01), and recombinant human or mouse cytokine factors (rh or rm) that inhibit cell differentiation.