Maintenance and Neuronal Differentiation of Chicken Induced Pluripotent Stem-Like Cells
Table 1
Ingredients of the five passaging media conditions presented in this study.
Media name
BRL-C. plus
cESC
2i+
cESC 2i+
2i+ mod.
KO DMEM
82%
87%
82%
87%
82%
FBS
15%
10%
15%
10%
15%
Na Pyruvate (100 mM)
1 mM
1 mM
1 mM
1 mM
1 mM
Glutamax (100x)
2 mM
1 mM
1 mM
1 mM
1 mM
Nonessential amino acids (100x)
1 mM
1 mM
1 mM
1 mM
1 mM
Beta-mercaptoethanol
0.005 mM
0.005 mM
0.005 mM
0.005 mM
0.005 mM
Penicillin/Streptomycin (100x)
1 mM
1 mM
1 mM
1 mM
1 mM
Nucleoside (100x)
1 mM
—
—
—
—
LIF
—
1000 U/mL
1000 U/mL
1000 U/mL
50 U/mL
PD0325901
—
—
0.5 μM
0.5 μM
1 μM
CHIR99021
—
—
3 μM
3 μM
3 μM
A83-01
—
—
0.5 μM
0.5 μM
1 μM
rhIL6
—
1 ng/mL
—
1 ng/mL
—
rhsILR6a
—
1 ng/mL
—
1 ng/mL
—
rmSCF
—
1 ng/mL
—
1 ng/mL
—
rhIGF1
—
1 ng/mL
—
1 ng/mL
—
rhFGF2
—
1 ng/mL
—
1 ng/mL
—
The media are all similar in a set of standard cell culture media ingredients (from KO DMEM to Penicillin/Streptomycin), but differ in the presence and concentrations of some of those ingredients, as well as LIF, 2i+ inhibitors (PDO325901, CHIR99021, and A83-01), and recombinant human or mouse cytokine factors (rh or rm) that inhibit cell differentiation.