Stem Cells International / 2014 / Article / Tab 1 / Research Article
Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model Table 1 Primers used for real-time PCR amplification.
Gene GenBank accession number Forward primer (5′-3′) Reverse primer (5′-3′) PCR product size (bp) eNOS NM_021838-2 TGGAAATTAACGTGGCTGTG GCCTTCTGCTCATTTTCCAA 112 ET-1 M64711.1 TGTCTACTTCTGCCACCTGGA CCTAGTCCATACGGGACGAC 69 TNF-α BC107671.1 GTCTACTGAACTTCGGGGTGA ATGAGAGGGAGCCCATTTG 67 MMP-9 NM_031055.1 AAAAGGCATCCAGCATCTGT AGCTGTCGGCTGTGGTTC 88 α -MHCNM_017239.2 CGAAACTGAAAACGGCAAG TGGCCATGTCCTCGATCT 92 β -MHCNM_017240.1 GCTGCAGAAGAAGCTCAAAGA GCAGCTTCTCCACCTTGG 98 Bax NM_017059.1 GTGAGCGGCTGCTTGTCT GACTCCAGCCACAAAGATGG 107 Caspase 3 NM_012922.2 AAACCTCCGTGGATTCAAAA AGCCCATTTCAGGGTAATCC 123 Bcl-2 NM_016993.1 GGGATGCCTTTGTGGAACT CTGAGCAGCGTCTTCAGAGA 82 α -actinU19893-1 CTGGGGCCTGAGGAGTTC CCGGTTGAACTCAGCATCA 87
eNOS = endothelial nitric oxide synthase; ET-1 = endothelin-1; TNF-α = tumor necrotic factor alpha; MMP = matrix metalloproteinase; MHC = myosin heavy chain; PCR = polymerase chain reaction.