Research Article
Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos
Table 1
Sequences of primers specific for microsatellite markers.
| Locus | Primer sequence (5′→3′) | Conditions | Denaturation | Annealing | Number of cycles | Extension |
| D2Mit296 | CAACTGTAAATCCAGTCGTAGGG CTCTGCTGAGGTTACTGTGGG | 94°C, 30 sec | 55°C, 30 sec | 40 | 5 min |
| D3Mit51 | GGCACTGATAGCAGGCCTAG TCTCTTCTGGTATTTCCTTCCG | 94°C, 60 sec | 55°C, 60 sec | 25 | 10 min |
| D11Mit20 | CCTGTCCAGGTTTGAGAGGA CTTGGGAGCCTCTTCGGT | 94°C, 60 sec | 55°C, 60 sec | 35 | 10 min |
| D13Mit88 | ACTGATGGCTCATGAGACCC AAAATTAATAGGAACTGCAAGGG | 94°C, 30 sec | 55°C, 30 sec | 40 | 5 min |
| D16Mit139 | GTATGTAAGGAATGGTCAAATTCTTG TCATTGTGATTGTGAAAGAATGC | 94°C, 30 sec | 55°C, 30 sec | 40 | 5 min |
| D19Mit10 | GCCTTTAAGCCAGTCAAGACA CCAGTCTGGACTTGTGAATGA | 94°C, 3 sec | 55°C, 30 sec | 40 | 5 min |
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