Research Article
Directing Parthenogenetic Stem Cells Differentiate into Adipocytes for Engineering Injectable Adipose Tissue
Table 1
The primers sequence for RT-PCR.
| Gene | Primers | Product |
| Snai1 | GACCTGTGGAAAGGCCTTCTCTAGG | 170 bp | CCTGGCACTGGTATCTCTTCACATC | Snai2 | GGGAGCATACAGCCCTATTACTG | 146 bp | CCTTGGATGAAGTGTCAGAGGAA | Hand1 | GCTACGCACATCATCACCATCATC | 125 bp | CAGCAGCCAGCTCTGGAAGTAAG | GATA2 | GCCAAAAGAGAGACTGGAGGAAGGG | 82 bp | ACACCTCCCACCTTTTAGTCACTCTG | Meox1 | CAGTCAAAATGTTCAGCATGGTAG | 195 bp | AGAGGAAAATGTTGAATGGAAACTTTA | Myf5 | CCTGAAGAAGGTCAACCAAGCTTTC | 158 bp | GGCTGTAATAGTTCTCCACCTGTTC | PAX3 | CTCTGAACCTGATTTACCGCTGAA | 124 bp | CCTGGTGTAAATGTCTGGGTAGTG | Pax7 | CCTCAGGTCATGAGCATCCTTAGC | 192 bp | GTAGGTGGGTGGGCAGTAAGACTG | Chrd | GGTGCAAGTGGTAGGTACAGGTAG | 182 bp | GCTCGTTCTGTAGCAGCATATGAG | SHH | CCCAATTACAACCCCGACATCATATTTAAG | 196 bp | CCTCATAGTGTAGAGACTCCTCTGAATG | PAX2 | CTGTCCCTAATGGAGACTCCCAGA | 162 bp | CCTGTTCTGATTTGATGTGCTCTGATG | OSR1 | GCCACTTCACTAAGTCCTATAACCTAC | 169 bp | TTCCCACACTCTTGACACTTGAAA | α-fetoprotein | CCCTCATCCTCCTGCTACATTTC | 145 bp | GGAACAAACTGGGTAAAGGTGATG | β-catenin | GCAACCCTGAGGAAGAAG | 501 bp | TCCCAGCAGTACAACGAG | CXCR4 | CCACGGAGTCAGAATCCTCCAGTT | 572 bp | CCAGCATTTCTACCACCATTTCAGG | NCAM1 | CGACTCCTCTACCCTCACCATCT | 559 bp | CTCGTCATCTTCCTCCTCGTTCT | NESTIN | GTTACCAAAGCCTCTTAGAAATGACC | 577 bp | CAGATGCAACTCTGCCTTATCCTC | NOTCH1 | AATGTGGATGCTGCTGTTGTGCT | 569 bp | ATGGATGGAGACTGCTGGAATGG | NANOG | GCACTCAAGGACAGGTTTCAG | 169 bp | GACCATTGCTAGTCTTCAACCAC | OCT3/4 | GTGTGAGGTGGAGTCTGGAG | 182 bp | AGCCTCATACTCTTCTCGTTGG | β-actin | GAGACAACATTGGCATGGCTTTG | 190 bp | CCTCAGCCACATTTGTAGAACTTTG |
|
|