HLA-E: Presentation of a Broader Peptide Repertoire Impacts the Cellular Immune Response—Implications on HSCT Outcome

Figure 1

Noncanonical peptides stabilize HLA-E surface expression. The T2 cell line transduced with the 01:01 allele was incubated in the presence or absence of the indicated peptides at 37°C for 2.5 hours prior to staining with the mab 3D12 (anti-HLA-E) and analyzed by flow cytometry. Each peptide was titrated with the indicated concentrations and compared to a known HLA-E peptide ligand VMAPRTLFL that was carried along with a saturated concentration of 200 μM indicated by the difference in median fluorescence intensity between T2E cells in the presence of peptide ± SEM of at least two individual experiments. FI values from T2E cells in the absence of peptide were subtracted from experimental samples as background control.