Research Article
HLA-E: Presentation of a Broader Peptide Repertoire Impacts the Cellular Immune Response—Implications on HSCT Outcome
Figure 2
Noncanonical peptides impact on protection against NK cell cytotoxicity. (a) T2E cells were incubated in the presence or absence of peptides prior a 4-hour cytotoxicity assay with NK cells (NKL) or fresh isolated untouched CD56+ NK cells from PBMCs at a E : T ratio of 10 : 1 and (b) untouched NK cells at 10 : 1 or 50 : 1. Specific cell death was calculated based on the percentages of dead (7-AAD+) and live cells (CFSE+) ± SEM. Specific cell death of target cells incubated with peptide was compared to target cells without peptide incubation.
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