Review Article

Prospect of Human Pluripotent Stem Cell-Derived Neural Crest Stem Cells in Clinical Application

Table 1

A summary of studies about NCSC differentiation from human pluripotent stem cells.

ReferenceCell sourceCulture systemSupplemental factorsThe yield of NCSCsTime lengthCells induced from NCSCs in vitroIn vivo experimentRemarks

Pomp et al., 2005 [16]hESCsCoculture
(PA6)
Serum42.3%  ±  5.5%1 weekPeripheral sensory and sympathetic neuronsNullFirst paper about NCSC induction from hESCs, but only a few molecular markers were tested

Lee et al., 2007 [17]hESCsCoculture
(MS-5)
Culture were switched to N2 media with serum after neural rosettes emerge from the differentiating hESCs30%1 monthPeripheral nerve system and mesenchymal stem cellsChicken embryos and adult miceFirst report using in vivo transplantation to confirm the identity of hESC-derived NCSCs, which were capable of self-renewal for several passages

Jiang et al., 2009 [18]hESCsCoculture
(PA6)
Serum55.3%  ±  10.6% of the hESC colonies expressing P751 weekPeripheral neurons, glial cells, and myofibroblastsChicken embryosP75+ cells after sorting showed self-renewal capacity

Hotta et al., 2009 [19]hESCsCoculture
(MEF)
Noggin, Y2763297.7%  ±  0.7% of the migrating cells were P75+20 daysEnteric neuronsQuail embryosFirst study reporting the use of Y27632 to induce hESCs into migrating NCSCs

Lee et al., 2010 [20]hESCs
hiPSCs
() Coculture
(MS-5)
() 2D monolayer
(Matrigel)
Noggin, SB431542 in defined media() 30%
() 15%40%
() 30 days
() 12 days
Peripheral neurons, Schwann cells, and myofibroblastsNullA feeder-free system was developed to generated NCSCs from both hESCs and hiPSCs

Menendez et al., 2011 [21]hESCs
hiPSCs
2D monolayer
(Geltrex)
SB431542, BIO80%99%2 weeksPeripheral neurons and mesenchymal stem cellsChicken embryosThis one-step protocol not only is efficient, but also generates NCSCs with stable self-renewal capacity for over 30 passages

Liu et al., 2012 [22]hESCs
hiPSCs
2D monolayer
(Geltrex)
PA6 conditioned media containing FGF2, Rock inhibitor, and ascorbic acid46%14 daysPeripheral neurons, Schwann cells, and mesenchymal lineagesChicken embryosFirst report showed in vitro myelination ability of Schwann cells differentiated from hESC/hiPSC-derived NCSCs

MEF: mouse embryonic fibroblasts; FGF2: fibroblast growth factor 2.