Research Article

Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model

Figure 3

Arrangement of hESC-MSC-Fbs at the wound site after three ischemia/reperfusion (I/R) cycles. (a) Wounded skin after treatment with phosphate-buffered saline (PBS), human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs), or hESC-MSC-derived fibroblasts (hESC-MSC-Fbs) at 12 days after three I/R cycles was observed under fluorescent microscopy for the detection of DiI. (b) Wounded skin after treatment with PBS, hESC-MSCs, or hESC-MSC-Fbs at 12 days after three I/R cycles was immunostained for the detection of α-SMA. 4′,6′-Diamidino-2-phenylindole was used for nuclear counterstaining.
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