Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model

Table 1

(a)

Role of genes	Genes	Origin	F/R	Primer sequences
MSC marker	CD90	Human	Forward	ATGAAGGTCCTCTACTTATCCGC
			Reverse	GCACTGTGACGTTCTGGGA
	CD44	Human	Forward	CTGCCGCTTTGCAGGTGTA
			Reverse	CATTGTGGGCAAGGTGCTATT
	CD29	Human	Forward	CAAGAGAGCTGAAGACTATCCCA
			Reverse	TGAAGTCCGAAGTAATCCTCCT
Fibroblastic hallmarks	Tn-C	Human	Forward	TCCCAGTGTTCGGTGGATCT
			Reverse	TTGATGCGATGTGTGAAGACA
	Col I	Human	Forward	GAGGGCCAAGACGAAGACATC
			Reverse	CAGATCACGTCATCGCACAAC
	Col III	Human	Forward	GCCAAATATGTGTCTGTGACTCA
			Reverse	GGGCGAGTAGGAGCAGTTG
	FN 1	Human	Forward	CGGTGGCTGTCAGTCAAAG
			Reverse	AAACCTCGGCTTCCTCCATAA
	FSP1	Human	Forward	GATGAGCAACTTGGACAGCAA
			Reverse	CTGGGCTGCTTATCTGGGAAG
	MMP-1	Human	Forward	GGGGCTTTGATGTACCCTAGC
			Reverse	TGTCACACGCTTTTGGGGTTT
Housekeeping gene	β-Actin	Human	Forward	TCCCTGGAGAAGAGCTACGA
			Reverse	AGCACTGTGTTGGCGTACAG

(b)

Role of gene	Gene	Origin	F/R	Primer sequences (5-3)
Inflammation	IL-10	Mouse	Forward	CCAAGCCTTATCGGAAATGA
			Reverse	TTTTCACAGGGGAGAAATCG
	IL-6	Mouse	Forward	CCGGAGAGGAGACTTCACAG
			Reverse	CAGAATTGCCATTGCACAAC
	IL-1β	Mouse	Forward	TCCCAAGCAATACCCAAAGAAGAA
			Reverse	TGGGGAAGGCATTAGAAACAGTC
	IL-12β	Mouse	Forward	TGGTTTGCCATCGTTTTGCTG
			Reverse	ACAGGTGAGGTTCACTGTTTCT
Angiogenesis	PECAM-1	Mouse	Forward	ACGGTCTTGTCGCAGTATCA
			Reverse	TGGGTGCAGTTCCATTTTCG
	VCAM-1	Mouse	Forward	CAGCTAAATAATGGGGAACTG
			Reverse	GACGGTGTCTCCCTCTTTGA
Housekeeping gene	β-Actin	Mouse	Forward	AGTGTGACGTTGACATCCGT
			Reverse	TGCTAGGAGCCAGAGCAGTA