Research Article

Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model

Table 1

(a)

Role of genesGenesOriginF/RPrimer sequences

MSC markerCD90HumanForwardATGAAGGTCCTCTACTTATCCGC
ReverseGCACTGTGACGTTCTGGGA
CD44HumanForwardCTGCCGCTTTGCAGGTGTA
ReverseCATTGTGGGCAAGGTGCTATT
CD29HumanForwardCAAGAGAGCTGAAGACTATCCCA
ReverseTGAAGTCCGAAGTAATCCTCCT

Fibroblastic hallmarksTn-CHumanForwardTCCCAGTGTTCGGTGGATCT
ReverseTTGATGCGATGTGTGAAGACA
Col IHumanForwardGAGGGCCAAGACGAAGACATC
ReverseCAGATCACGTCATCGCACAAC
Col IIIHumanForwardGCCAAATATGTGTCTGTGACTCA
ReverseGGGCGAGTAGGAGCAGTTG
FN 1HumanForwardCGGTGGCTGTCAGTCAAAG
ReverseAAACCTCGGCTTCCTCCATAA
FSP1HumanForwardGATGAGCAACTTGGACAGCAA
ReverseCTGGGCTGCTTATCTGGGAAG
MMP-1HumanForwardGGGGCTTTGATGTACCCTAGC
ReverseTGTCACACGCTTTTGGGGTTT

Housekeeping geneβ-ActinHumanForwardTCCCTGGAGAAGAGCTACGA
ReverseAGCACTGTGTTGGCGTACAG

(b)

Role of geneGeneOriginF/RPrimer sequences (5-3)

InflammationIL-10MouseForwardCCAAGCCTTATCGGAAATGA
ReverseTTTTCACAGGGGAGAAATCG
IL-6MouseForwardCCGGAGAGGAGACTTCACAG
ReverseCAGAATTGCCATTGCACAAC
IL-1βMouseForwardTCCCAAGCAATACCCAAAGAAGAA
ReverseTGGGGAAGGCATTAGAAACAGTC
IL-12βMouseForwardTGGTTTGCCATCGTTTTGCTG
ReverseACAGGTGAGGTTCACTGTTTCT

AngiogenesisPECAM-1MouseForwardACGGTCTTGTCGCAGTATCA
ReverseTGGGTGCAGTTCCATTTTCG
VCAM-1MouseForwardCAGCTAAATAATGGGGAACTG
ReverseGACGGTGTCTCCCTCTTTGA

Housekeeping geneβ-ActinMouseForwardAGTGTGACGTTGACATCCGT
ReverseTGCTAGGAGCCAGAGCAGTA