Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor

Figure 1

(a) Differentiation protocols for the derivation of ATII using an embryoid body seeding method and a stepwise differentiation method. (b) Quantitative polymerase chain reaction analysis of DE (day 7) (). Expression of Cxcr4, Foxa2, and Sox17 mRNA. Expression ratios were normalized to the level of Gapdh expression. Expression levels were compared with those of undifferentiated iPSCs (day 0). . (c) Phase-contrast image and immunofluorescence images of FOXA2 in DE (day 7). Scale bars: 100 μm. (d) Quantitative polymerase chain reaction analysis of AFE (day 9) (). Expression of Pax9, Tbx1, and Sox2 mRNA. Expression ratios were normalized to the level of Gapdh expression. Expression levels were compared with those of undifferentiated iPSCs (day 0). . (e) Phase-contrast image and immunofluorescence images of PAX9 and SOX2 in AFE (day 9). Scale bars: 100 μm.

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