Research Article

Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor

Figure 3

(a) Quantitative polymerase chain reaction analysis of ATII (day 26) (). Expressions of Sftpc and Sftpb mRNA. Expression ratios were normalized to the level of Gapdh expression. Expression levels were compared with those of ATII (day 26). . (b) Phase-contrast image and immunofluorescence images of pro-SPC and pro-/mature SPB in ATII (day 26). Scale bars: 100 μm. (c) Enzyme-linked immunosorbent assay of SPC in culture supernatant (). . ND: not detected. (d) Quantitative polymerase chain reaction analysis of ATII (day 26) (). Expressions of Nkx2-1 mRNA. Expression ratios were normalized to the level of Gapdh expression. Expression levels were compared with those of VAFE (day 16). ND: not detected. (e) Flow cytometry analysis of pro-SPC in ATII (day 26).
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