Research Article
Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor
Figure 4
(a) Effect of JQ1 on the WST-1 reduction potential in undifferentiated iPSCs (). Undifferentiated iPSCs were treated with 0, 0.05, 0.1, 0.2, 0.5, 1.0, or 2.0 μM JQ1. . (b) Effect of JQ1 on WST-1 reduction potential in MLE12 (). Undifferentiated iPSCs were treated with JQ1 at 0, 0.05, 0.1, 0.2, 0.5, 1.0, or 2.0 μM. (c) Quantitative polymerase chain reaction analysis in undifferentiated iPSCs (n = 4). Expressions of Klf4, cMyc, Pou5f1, and Sox2 mRNA. Expression ratios were normalized to the level of Gapdh expression. Expression levels were compared with those of undifferentiated cells (day 0). . (d) Quantitative polymerase chain reaction analysis (). Expressions of Pou5f1 and Sox2 mRNA. Expression ratios were normalized to the level of Gapdh expression. Pou5f1 and Sox2 mRNA expression levels were decreased. (e) Western blot analysis of undifferentiated iPSCs, with the expression of BRD4 protein shown.
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