Activator Protein-1 Transcriptional Activity Drives Soluble Micrograft-Mediated Cell Migration and Promotes the Matrix Remodeling Machinery

Figure 5

Transcriptional and posttranscriptional regulation of the matrix remodeling machinery via MG-dependent AP-1 activity. (a) Matrix metalloproteinase (MMP) gene expression levels were evaluated by RT-qPCR in 5 h MG-treated adult fibroblasts in the presence or absence of T-5224 (60 μM). Expression values are expressed as -score of the average fold change (FC) normalized to the expression of GAPDH, β-actin, and Rpl13a genes (). (b) Enzymatic activity of MMPs in adult fibroblasts exposed to 5 and 12 h of MG treatment was evaluated via FRET (fluorescence resonance energy transfer). Signal intensities were normalized to the substrate control. Data is reported as relative fluorescence units (RFU). (c) MMP gene expression levels were evaluated by RT-qPCR in 5 h and 12 h MG-treated human keratinocytes in the presence or absence of T-5224 (60 μM). Expression values are expressed as -score of the average FC normalized on the expression of GAPDH, β-actin, and RPL13A genes (). (d) Enzymatic activity of matrix metalloproteinases of conditioned media derived from human keratinocytes exposed to 5 and 12 h of MG treatment was evaluated via FRET (frequency resonance energy transfer). Signal intensity signals were normalized to the substrate control. Data is reported as relative fluorescence units (RFU).

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