Interleukin-1β Enhances Umbilical Cord Mesenchymal Stem Cell Adhesion Ability on Human Umbilical Vein Endothelial Cells via LFA-1/ICAM-1 Interaction

Figure 3

Effects of cycloheximide in MSC adhesion to HUVECs. (a) Western blot results of the LFA-1 (129 kDa) from membrane protein. Cells treated with IL-1β and cycloheximide (CYH) or cotreated with both IL-1β and CYH. (b) Quantitative graphs of the Western blot results of LFA-1 protein expression of (a). (, , and ). (c) Representative image of MSC adhesion on HUVECs. MSCs treated with IL-1β; cycloheximide adhesion to IL-1β-activated HUVECs or nonactivated HUVECs. MSCs labelled with calcein AM (5 μM) (green), HUVECs, and MSC cell nuclei were stained with Hoechst 33258 (blue) (scale bar: 50 μm). (d) Quantitative graphs of the cell adhesion assay results of MSC adhesion to nonactivated HUVECs (black bars) or IL-1β-activated HUVECs (gray bars). MSCs treated with IL-1β and cycloheximide. MSC adhesion cell number counted in five randomly selected fields in a single representative experiment performed three times. Values were the cell number fold change relative to the control group. Data represent (, , and ) (N.S.: nonsignificance).

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